Application
alpha Actinin 1 antibody detects alpha Actinin 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-actinin 1 antibody at 1:100 dilution.| Product Name | alpha Actinin 1 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Actinin 1 (actinin, alpha 1)Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Actinin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Chicken, BovineRat (98%), Zebrafish (98%), Xenopus laevis (97%), Pig (98%), Chicken (97%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ40884 antibody FLJ54432 antibody ACTN1 antibody alpha-actinin-1 antibody F-actin cross-linking protein antibody actinin 1 smooth muscle antibody alpha-actinin cytoskeletal isoform antibody non-muscle alpha-actinin-1 antibody "actinin alpha 1 an |
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| Molecular Weight(MW) | 103 kDa (note) (3) |
| Cellular Localization | Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk |
Application
alpha Actinin 1 antibody detects alpha Actinin 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-actinin 1 antibody at 1:100 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
