Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:3000Application
alpha Actinin 2 antibody [N2C1], Internal detects alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. alpha Actinin 2 antibody [N2C1], Internal diluted at 1:250.Application
alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody diluted at 1:5000.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:1000| Product Name | alpha Actinin 2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Actinin 2 (actinin, alpha 2)Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (89%), Pig (99%), Chicken (97%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMD1AA antibody ACTN2 antibody F-actin cross-linking protein antibody alpha-actinin-2 antibody alpha-actinin skeletal muscle antibody "actinin alpha 2 antibody" |
|---|---|
| Molecular Weight(MW) | 104 kDa (note) (3) |
| Cellular Localization | Cytoplasm , myofibril , sarcomere , Z-disk |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:3000
Application
alpha Actinin 2 antibody [N2C1], Internal detects alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. alpha Actinin 2 antibody [N2C1], Internal diluted at 1:250.
Application
alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody diluted at 1:5000.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 , Rat heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
