Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE diluted at 1:10000Application
alpha Actinin 3 antibody detects alpha Actinin 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.Application
Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 μg of alpha Actinin 3 antibody . Western blot analysis was performed using alpha Actinin 3 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: PC-12 7.5% SDS PAGE diluted at 1:10000| Product Name | alpha Actinin 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Actinin 3 (alpha-actinin-3)Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Actinin 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, BovineXenopus laevis (94%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC117002 antibody MGC117005 antibody ACTN3 antibody F-actin cross-linking protein antibody alpha-actinin-3 antibody alpha-actinin skeletal muscle antibody "actinin alpha 3 antibody" |
|---|---|
| Molecular Weight(MW) | 103 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE diluted at 1:10000
Application
alpha Actinin 3 antibody detects alpha Actinin 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.
Application
Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 μg of alpha Actinin 3 antibody . Western blot analysis was performed using alpha Actinin 3 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: PC-12 7.5% SDS PAGE diluted at 1:10000| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
