Application
Immunofluorescence staining of methanol-fixed Hela cells using ADD1 (Phospho-Ser726) Antibody .Application
Western blot analysis of extracts from Hela cells using ADD1(Phospho-Ser726) Antibody and the same antibody preincubated with blocking peptide.Application
Western blot analysis of extracts from Hela cells using ADD1 (Phospho-Ser726) Antibody and the same antibody preincubated with blocking peptide.Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Phospho-Ser726) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | alpha Adducin (phospho Ser726) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Adducin (phospho Ser726)Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
| Immunogen | Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1. |
| Modification | p-Ser726 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADDA MGC3339 MGC44427 ADD1 alpha-adducin erythrocyte adducin alpha subunit adducin 1 (alpha) |
|---|---|
| Target | alpha Adducin (phospho Ser726) |
| Molecular Weight(MW) | 130 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using ADD1 (Phospho-Ser726) Antibody .
Application
Western blot analysis of extracts from Hela cells using ADD1(Phospho-Ser726) Antibody and the same antibody preincubated with blocking peptide.
Application
Western blot analysis of extracts from Hela cells using ADD1 (Phospho-Ser726) Antibody and the same antibody preincubated with blocking peptide.
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Phospho-Ser726) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
