Application
Immunofluorescence staining of methanol-fixed Hela cells using ADD1 Antibody .Application
Western blot analysis of extracts from 293 and Hela cells using ADD1 Antibody .Application
Western blot analysis of extracts from 293 and Hela cells using ADD1(Ab-726) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | alpha Adducin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ADD1Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
| Immunogen | Peptide sequence around aa.724~728 (T-P-S-F-L) derived from Human ADD1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADDA MGC3339 MGC44427 ADD1 alpha-adducin erythrocyte adducin alpha subunit adducin 1 (alpha) |
|---|---|
| Target | ADD1 |
| Molecular Weight(MW) | 130 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using ADD1 Antibody .
Application
Western blot analysis of extracts from 293 and Hela cells using ADD1 Antibody .
Application
Western blot analysis of extracts from 293 and Hela cells using ADD1(Ab-726) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
