Application
alpha Adducin antibody detects alpha Adducin protein at membrane and cytosol on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. alpha Adducin antibody dilution: 1:500.Application
alpha Adducin antibody detects alpha Adducin protein at membrane and cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. alpha Adducin antibody dilution: 1:500.Application
alpha Adducin antibody detects alpha Adducin protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract 7.5% SDS-PAGE alpha Adducin antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Adducin antibody detects alpha Adducin protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: alpha Adducin protein stained by alpha Adducin antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | alpha Adducin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Adducin (adducin 1 (alpha))Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Adducin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (97%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADD1 ADDA adducin 1 (alpha) |
|---|---|
| Target | alpha Adducin |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell membrane |
Application
alpha Adducin antibody detects alpha Adducin protein at membrane and cytosol on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. alpha Adducin antibody dilution: 1:500.
Application
alpha Adducin antibody detects alpha Adducin protein at membrane and cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. alpha Adducin antibody dilution: 1:500.
Application
alpha Adducin antibody detects alpha Adducin protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract 7.5% SDS-PAGE alpha Adducin antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Adducin antibody detects alpha Adducin protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: alpha Adducin protein stained by alpha Adducin antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Positive Control | Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
