| Product Name | alpha Amylase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal to Human Alpha - AmylaseAmylase catalyses the hydrolysis of internal 1,4-glucan links in large linear polymers at internal bonds. The enzyme is present in all living organisms with variable enzymatic activity. |
| Immunogen | Highly purified human alpha-amylase |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-FIHC-PWB |
| For IHC-Fr: Use at 1:100-1:150. For IHC-P: Use at 1:100-1:150. Prolonged fixation in buffered formalin can destroy the epitope. For WB: Use at 1:200-1:500. For ELISA: Use at 1:500-1:1000. Optimal dilutions/concentrations should be determined by the end user. Some researchers have sucessfully used this product in IE, immunodiffusion and precipitation, but has not yet been tested in house.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMY1 AMY1B AMY1C |
|---|---|
| Target | alpha Amylase |
| Tissue Specificity | This antiserum is determined to be immunospecific for human a-amylase found in human saliva and human pancreatic extract by Ouchterlony double diffusion and immunoelectrophoresis. No reaction with other human saliva proteins or pancreatic proteins is observed. |
| Positive Control | Pancreas |
|---|---|
| Application Notes | For IHC-Fr: Use at 1:100-1:150. For IHC-P: Use at 1:100-1:150. Prolonged fixation in buffered formalin can destroy the epitope. For WB: Use at 1:200-1:500. For ELISA: Use at 1:500-1:1000. Optimal dilutions/concentrations should be determined by the end user. Some researchers have sucessfully used this product in IE, immunodiffusion and precipitation, but has not yet been tested in house.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.05% sodium azide |
