Application
alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein by western blot analysis. Various whole cell extracts (30 µg) and Rat tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Cardiac Muscle Actin antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE alpha Actin (cardiac muscle) antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody diluted at 1:200. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using alpha Actin (cardiac muscle) antibody at 1:100 dilution.Application
Sample(30 µg of whole cell lysate) A:HeLa S3 B:MOLT4 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | alpha Cardiac Muscle Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Cardiac Muscle Actin (actin, alpha, cardiac muscle 1)Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Cardiac Muscle Actin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (99%), Pig (100%), Chicken (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACTC antibody ASD5 antibody CMD1R antibody CMH11 antibody LVNC4 antibody ACTC1 antibody "actin alpha cardiac muscle 1 antibody" alpha-cardiac actin antibody "actin alpha cardiac muscle 1 antibody" |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein by western blot analysis. Various whole cell extracts (30 µg) and Rat tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Cardiac Muscle Actin antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE alpha Actin (cardiac muscle) antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using alpha Actin (cardiac muscle) antibody at 1:100 dilution.
Application
Sample(30 µg of whole cell lysate) A:HeLa S3 B:MOLT4 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , Raji , K562 , THP-1 , NIH-3T3 , JC , BCL-1 , Rat muscle , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
