Application
Immunofluorescence of AFP in rat liver tissue with AFP antibody at 20 ug/mL.Application
Western blot analysis of AFP in fetal human liver tissue lysate with AFP antibody at (A) 1 ug/mL and (B) 2 ug/mL.Application
Immunohistochemistry of AFP in rat liver tissue with AFP antibody at 10 ug/mL.| Product Name | Alpha fetoprotein / AFP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Alpha fetoprotein / AFPThis gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008] |
| Immunogen | AFP antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human AFP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 ug/ml. IHC-P: 10 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AFPD HPAFP alpha-fetoprotein FETA AFP |
|---|---|
| Target | Alpha fetoprotein / AFP |
Application
Immunofluorescence of AFP in rat liver tissue with AFP antibody at 20 ug/mL.
Application
Western blot analysis of AFP in fetal human liver tissue lysate with AFP antibody at (A) 1 ug/mL and (B) 2 ug/mL.
Application
Immunohistochemistry of AFP in rat liver tissue with AFP antibody at 10 ug/mL.| Application Notes | WB: 1 ug/ml. IHC-P: 10 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
