Application
alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody diluted by 1:1000.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody diluted at 1:1000.Application
Immunoprecipitation of alpha Internexin protein from U87-MG whole cell extracts using 5 μg of alpha Internexin antibody . Western blot analysis was performed using alpha Internexin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody diluted at 1:1000.Application
alpha Internexin antibody detects alpha Internexin protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: alpha Internexin protein stained by alpha Internexin antibody diluted at 1:250. Blue: Fluoroshield with DAPI .Application
alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody diluted by 1:1000.| Product Name | alpha Internexin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Internexin (internexin neuronal intermediate filament protein, alpha)Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha Internexin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NF-66 TXBP-1 NEF5 internexin neuronal intermediate filament protein alpha INA |
|---|---|
| Target | alpha Internexin |
| Molecular Weight(MW) | 55 kDa (note) |
Application
alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody diluted by 1:1000.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody diluted at 1:1000.
Application
Immunoprecipitation of alpha Internexin protein from U87-MG whole cell extracts using 5 μg of alpha Internexin antibody . Western blot analysis was performed using alpha Internexin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody diluted at 1:1000.
Application
alpha Internexin antibody detects alpha Internexin protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: alpha Internexin protein stained by alpha Internexin antibody diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody diluted by 1:1000.| Positive Control | U87-MG , SK-N-SH , SK-N-AS , SH-SY5Y , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
