Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:1000Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Sarcomeric Actin antibody diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded human Spleen, using gamma Actin antibody(10 μg/ml).Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000| Product Name | alpha Sarcomeric Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Sarcomeric Actin (actin, cytoplasmic 2)Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human alpha Sarcomeric Actin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Chicken, Chimpanzee, BovineXenopus laevis (100%), Dog (96%), Chicken (100%), Chimpanzee (94%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACT antibody ACTG antibody DFNA20 antibody DFNA26 antibody ACTG1 antibody cytoskeletal gamma-actin antibody "actin cytoplasmic 2 antibody" "actin gamma 1 antibody" |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:1000
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Sarcomeric Actin antibody diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded human Spleen, using gamma Actin antibody(10 μg/ml).
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000| Positive Control | A431 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
