Application
Sample(30 μg of whole cell lysate) A:Raji 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using alpha Actin (skeletal muscle) antibody(10 μg/ml).Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 10% SDS PAGE diluted at 1:5000| Product Name | alpha Skeletal Muscle Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Skeletal Muscle Actin (actin, alpha 1, skeletal muscle)The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Skeletal Muscle Actin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Japanese medaka, Xenopus laevis, Pig, Chicken, BovineRat (100%), Japanese medaka (98%), Xenopus laevis (98%), Pig (100%), Chicken (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACTA antibody ASMA antibody CFTD antibody CFTD1 antibody CFTDM antibody MPFD antibody NEM1 antibody NEM2 antibody NEM3 antibody ACTA1 antibody alpha-actin-1 antibody alpha skeletal muscle actin antibody "actin alpha skeletal muscle antibody" |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
Sample(30 μg of whole cell lysate) A:Raji 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using alpha Actin (skeletal muscle) antibody(10 μg/ml).
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 D: C2C12 10% SDS PAGE diluted at 1:5000| Positive Control | Raji , NIH-3T3 , JC , BCL-1 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
