Application
Western blot of 3T3 (A) and mouse heart whole tissue lysate (B) labeled with anti-Actin antibody alpha smooth muscle .Application
WB analysis of 3T3 lysate using alpha Smooth Muscle Actin antibody at a concentration of 1 ug/ml in the abscence (A) and prescence (B) of blocking peptide.Application
WB analysis of 3T3 lysate using alpha Smooth Muscle Actin antibody at a concentration of 1.0 μg/ml.Application
IHC analysis of human leiomyoma using alpha Smooth Muscle Actin antibody at a dilution of 1:100. Antigen retrieval was performed by boiling in 10 mM citrate buffer, pH 6.0 for 10 min.Application
Immunocytochemistry of human dermal collagen in the skin (Formalin fixed/Paraffin-embedded) stained with| Product Name | alpha Smooth Muscle Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to alpha Smooth Muscle ActinThe protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008] |
| Immunogen | Synthetic peptide (Human) corresponding to N-terminus of smooth muscle alpha actin. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| WB:1:500~1:2000 IP:1:100~1:500 ICC/IF:1:100~1:500 IHC:1:50~1:500 |
|
| Species Reactivity | BovineHumanMouseRatZebra Fish |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACTA2 ACTSA MYMY5 AAT6 alpha SMA antibody Smactin antibody alpha SM actin antibody ASMA antibody aortic smooth muscle Alpha-actin-2 antibody actin aortic smooth muscle antibody cell growth inhibiting gene 46 protein antibo |
|---|---|
| Target | alpha Smooth Muscle Actin |
| Tissue Specificity | This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. The other actins, such as beta- and gamma-cytoplasmic, striated muscle, and myocardium are not stained by this antibody. |
| Cellular Localization | Cytoplasmic |
Application
Western blot of 3T3 (A) and mouse heart whole tissue lysate (B) labeled with anti-Actin antibody alpha smooth muscle .
Application
WB analysis of 3T3 lysate using alpha Smooth Muscle Actin antibody at a concentration of 1 ug/ml in the abscence (A) and prescence (B) of blocking peptide.
Application
WB analysis of 3T3 lysate using alpha Smooth Muscle Actin antibody at a concentration of 1.0 μg/ml.
Application
IHC analysis of human leiomyoma using alpha Smooth Muscle Actin antibody at a dilution of 1:100. Antigen retrieval was performed by boiling in 10 mM citrate buffer, pH 6.0 for 10 min.
Application
Immunocytochemistry of human dermal collagen in the skin (Formalin fixed/Paraffin-embedded) stained with| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 ICC/IF:1:100~1:500 IHC:1:50~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS. pH 7.4 |
