Application
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha smooth muscle Actin antibody detects ACTA2 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE alpha smooth muscle Actin antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using alpha Actin (smooth muscle) antibody at 1:500 dilution.Application
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis. Sample: Paraffin-embedded liver carcinoma stroma. alpha smooth muscle Actin antibody dApplication
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. alpha smooth muscle Actin antibody dilution: 1:500.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | alpha Smooth Muscle Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to alpha Smooth Muscle ActinThe protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human alpha Smooth Muscle Actin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRatZebra Fish |
| Predicted Reactivity | Pig, Rabbit, Chicken, Rhesus Monkey, BovinePig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACTA2 ACTSA MYMY5 AAT6 alpha SMA antibody Smactin antibody alpha SM actin antibody ASMA antibody aortic smooth muscle Alpha-actin-2 antibody actin aortic smooth muscle antibody cell growth inhibiting gene 46 protein antibo |
|---|---|
| Target | alpha Smooth Muscle Actin |
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha smooth muscle Actin antibody detects ACTA2 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 10% SDS-PAGE alpha smooth muscle Actin antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using alpha Actin (smooth muscle) antibody at 1:500 dilution.
Application
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis. Sample: Paraffin-embedded liver carcinoma stroma. alpha smooth muscle Actin antibody d
Application
alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. alpha smooth muscle Actin antibody dilution: 1:500.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , C8D30 , NIH-3T3 , C2C12 , Rat2 , *Mouse Heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
