Application
Immunofluorescence analysis of methanol-fixed A431, using alpha SNAP antibody at 1:200 dilution.Application
alpha SNAP antibody [N2C3] detects alpha SNAP protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. alpha SNAP antibody [N2C3] dilution: 1:500.Application
Sample(30 ug whole cell lysate) A:Hep G2 10% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000| Product Name | alpha SNAP antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha SNAP (N-ethylmaleimide-sensitive factor attachment protein, alpha)The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37%, 60%, and 67% identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha SNAP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Rhesus Monkey, Chimpanzee, BovineXenopus laevis (89%), Pig (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SNAPA antibody NAPA antibody alpha-SNAP antibody alpha-soluble NSF attachment protein antibody "N-ethylmaleimide-sensitive factor attachment protein alpha antibody" |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) |
| Cellular Localization | Membrane; Peripheral membrane protein |
Application
Immunofluorescence analysis of methanol-fixed A431, using alpha SNAP antibody at 1:200 dilution.
Application
alpha SNAP antibody [N2C3] detects alpha SNAP protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. alpha SNAP antibody [N2C3] dilution: 1:500.
Application
Sample(30 ug whole cell lysate) A:Hep G2 10% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
