Application
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis. A. 30 µg NIH-3T3 whole cell extract B. 30 µg mouse brain extract 10% SDS-PAGE alpha Tubulin 1A antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis. A. 30 µg drosophila lysate/extract 10% SDS-PAGE alpha Tubulin 1A antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha 1A antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using alpha Tubulin 1A antibody at 1:500 dilution.Application
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis. A. 30 µg Jurkat whole cell extract B. 30 µg Raji whole cell extract C. 30 µg 293T whole cell extract D. 30 µg A431 whole cell extract E. 30 µg HeLa whole cell extract F. 30 µg HepG2 whole cell extract G. 30 µg H1299 whole cell extract H. 30 µg HCT116 whole cell extract I. 30 µg MCF-7 whole cell extract J. 30 µg NT2D1 whole cell extract K. 30 µg PC-3 whole cell extract L. 30 µg U87-MG whole cell extract 10% SDS-PAGE alpha Tubulin 1A antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis. A. 30 µg rat brain extract 10% SDS-PAGE alpha Tubulin 1A antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | alpha Tubulin 1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Tubulin 1A (tubulin, alpha 1a)Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 1A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseDrosophilaRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Rhesus Monkey, Chimpanzee, BovineZebrafish (100%), Xenopus laevis (98%), Pig (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B-ALPHA-1 antibody FLJ25113 antibody LIS3 antibody TUBA3 antibody TUBA1A antibody tubulin B-alpha-1 antibody hum-a-tub1 antibody "tubulin alpha brain-specific antibody" hum-a-tub2 antibody tubulin alpha-1A chain antibody "tubulin alpha 3 antib |
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| Molecular Weight(MW) | 50 kDa (note) (3) |
Application
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis. A. 30 µg NIH-3T3 whole cell extract B. 30 µg mouse brain extract 10% SDS-PAGE alpha Tubulin 1A antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis. A. 30 µg drosophila lysate/extract 10% SDS-PAGE alpha Tubulin 1A antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha 1A antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using alpha Tubulin 1A antibody at 1:500 dilution.
Application
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis. A. 30 µg Jurkat whole cell extract B. 30 µg Raji whole cell extract C. 30 µg 293T whole cell extract D. 30 µg A431 whole cell extract E. 30 µg HeLa whole cell extract F. 30 µg HepG2 whole cell extract G. 30 µg H1299 whole cell extract H. 30 µg HCT116 whole cell extract I. 30 µg MCF-7 whole cell extract J. 30 µg NT2D1 whole cell extract K. 30 µg PC-3 whole cell extract L. 30 µg U87-MG whole cell extract 10% SDS-PAGE alpha Tubulin 1A antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis. A. 30 µg rat brain extract 10% SDS-PAGE alpha Tubulin 1A antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , rat brain , Drosophila |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
