Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. alpha Tubulin 4a antibody diluted at 1:500.Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. alpha Tubulin 4a antibody diluted at 1:500.Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by western blot analysis. A. 30 µg Drosophila lysate/extract 10% SDS-PAGE alpha Tubulin 4a antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by western blot analysis. A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract 10% SDS-PAGE alpha Tubulin 4a antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin 4a protein stained by alpha Tubulin 4a antibody diluted at 1:1000. Blue: Hoechst 33342 staining.| Product Name | alpha Tubulin 4a antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Tubulin 4a (tubulin alpha-4A chain)Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 4a. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseChickenDrosophilaRat |
| Predicted Reactivity | Xenopus laevis, Sheep, Chimpanzee, BovineXenopus laevis (92%), Sheep (100%), Chimpanzee (99%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ30169 antibody, H2-ALPHA antibody, TUBA1 antibody, TUBA4A antibody, tubulin H2-alpha antibody, "tubulin, alpha 1 (testis specific) antibody", tubulin alpha-4A chain antibody, tubulin alpha-1 chain antibody, "tubulin, alpha 4a antibody" |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cytoskeleton , Microtubule |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. alpha Tubulin 4a antibody diluted at 1:500.
Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. alpha Tubulin 4a antibody diluted at 1:500.
Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 4a antibody diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by western blot analysis. A. 30 µg Drosophila lysate/extract 10% SDS-PAGE alpha Tubulin 4a antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein by western blot analysis. A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract 10% SDS-PAGE alpha Tubulin 4a antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
alpha Tubulin 4a antibody detects alpha Tubulin 4a protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin 4a protein stained by alpha Tubulin 4a antibody diluted at 1:1000. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 , Jurkat , Raji , HeLa cytosol fraction , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 , Drosophila |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
