Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using alpha Tubulin 8 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000Application
Sample (30 ug of whole cell lysate) A: A431 B: Hela 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin 8 antibody at 1:500 dilution.| Product Name | alpha Tubulin 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Tubulin 8 (tubulin, alpha 8)This gene encodes a member of the alpha tubulin protein family. Alpha tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene are associated with polymicrogyria and optic nerve hypoplasia. Alternate splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chimpanzee, BovineRat (99%), Xenopus laevis (85%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TUBAL2 antibody, TUBA8 antibody, tubulin alpha-8 chain antibody, tubulin alpha chain-like 2 antibody, "tubulin, alpha 8 antibody" |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using alpha Tubulin 8 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000
Application
Sample (30 ug of whole cell lysate) A: A431 B: Hela 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin 8 antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
