Application
alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha-1B antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | alpha Tubulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Tubulin (tubulin, alpha 1b)Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Rabbit, Rhesus Monkey, Chimpanzee, BovineRat (100%), Zebrafish (99%), Xenopus laevis (98%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | K-ALPHA-1 antibody, TUBA1B antibody, alpha-tubulin ubiquitous antibody, alpha tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-ubiquitous chain antibody, "tubulin, alpha, ubiquitous antibody", tubulin alpha-1B chain antibody, "tubulin, alpha 1b |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) |
Application
alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha-1B antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
