Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE ALPPL2 antibody diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed A549, using ALPPL2 antibody at 1:500 dilution.Application
ALPPL2 antibody detects ALPPL2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALPPL2 protein stained by ALPPL2 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human Breast, using ALPPL2 antibody at 1:500 dilution.| Product Name | ALPPL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ALPPL2 (alkaline phosphatase, placental-like 2)There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALPPL2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | EMICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALPG antibody ALPPL antibody GCAP antibody ALPPL2 antibody testicular and thymus alkaline phosphatase antibody placental-like alkaline phosphatase antibody "alkaline phosphatase placental-like antibody" alkaline phosphatase Nagao isozyme antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (2) |
| Cellular Localization | Cell membrane; Lipid-anchor , GPI-anchor |
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE ALPPL2 antibody diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed A549, using ALPPL2 antibody at 1:500 dilution.
Application
ALPPL2 antibody detects ALPPL2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALPPL2 protein stained by ALPPL2 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human Breast, using ALPPL2 antibody at 1:500 dilution.| Positive Control | A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
