Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:500| Product Name | AMPK gamma 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AMPK gamma 1 (5'-AMP-activated protein kinase subunit gamma-1)The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AMPK gamma 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Rhesus Monkey, BovineRat (98%), Zebrafish (86%), Xenopus laevis (80%), Pig (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMPKG antibody, MGC8666 antibody, PRKAG1 antibody, AMPK gamma1 antibody, "5'-AMP-activated protein kinase, gamma-1 subunit antibody", AMPK gamma-1 chain antibody, 5'-AMP-activated protein kinase subunit gamma-1 antibody, "protein kinase, AMP-activated, gam |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:500| Positive Control | mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
