Application
Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma Huh7 xenograft, using AGT antibody at 1:100 dilution.Application
Immunofluorescence analysis of methanol-fixed HepG2, using Angiotensinogen antibody at 1:200 dilution.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg whole cell lysate) A:Hep G2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Angiotensinogen antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Angiotensinogen (angiotensinogen (serpin peptidase inhibitor, clade A, member 8))The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Angiotensinogen. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | ChimpanzeeChimpanzee (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ANHU antibody, FLJ92595 antibody, FLJ97926 antibody, SERPINA8 antibody, AGT antibody, angiotensin II antibody, angiotensinogen antibody, serpin A8 antibody, angiotensin I antibody, serine (or cysteine) proteinase inhibitor antibody, "alpha-1 antiproteinase |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma Huh7 xenograft, using AGT antibody at 1:100 dilution.
Application
Immunofluorescence analysis of methanol-fixed HepG2, using Angiotensinogen antibody at 1:200 dilution.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiotensinogen antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg whole cell lysate) A:Hep G2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 , human plasma , mouse plasma , rat plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
