Application
Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ANKHD1 antibody at 1:100 dilution.Application
ANKHD1 antibody [N1], N-term detects ANKHD1 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE ANKHD1 antibody [N1], N-term dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE ANKHD1 antibody diluted at 1:1000| Product Name | ANKHD1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ANKHD1 (ankyrin repeat and KH domain containing 1)This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. Co-transcription of this gene and the neighboring downstream gene (EIF4EBP3) generates a transcript (MASK-BP3) which encodes a fusion protein comprised of the MASK protein sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ANKHD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, PigMouse (96%), Rat (95%), Dog (96%), Pig (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MASK antibody, VBARP antibody, ANKHD1 antibody, ankyrin repeat and KH domain-containing protein 1 antibody, HIV-1 Vpr-binding ankyrin repeat protein antibody, "multiple ankyrin repeats, single KH-domain homolog antibody", ankyrin repeat and KH domain conta |
|---|---|
| Molecular Weight(MW) | 269 kDa (note) (5) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ANKHD1 antibody at 1:100 dilution.
Application
ANKHD1 antibody [N1], N-term detects ANKHD1 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE ANKHD1 antibody [N1], N-term dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE ANKHD1 antibody diluted at 1:1000| Positive Control | A549 , HepG2 , HCT116 , Jurkat , Raji , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
