Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000Application
Antithrombin III antibody detects Antithrombin III protein at cytoplasm and nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. Antithrombin III antibody diluted at 1:250.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Antithrombin III antibody at 1:500 dilution.| Product Name | Antithrombin III antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Antithrombin III (antithrombin-III)The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Antithrombin III. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Sheep, Rhesus Monkey, Bovine, Guinea pigMouse (88%), Rat (86%), Pig (87%), Sheep (86%), Rhesus Monkey (91%), Bovine (84%), Guinea pig (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AT3 antibody, ATIII antibody, MGC22579 antibody, SERPINC1 antibody, serpin C1 antibody, antithrombin III antibody, antithrombin-III antibody, serine-cysteine proteinase inhibitor clade C member 1 antibody, "serine (or cysteine) proteinase inhibitor, clade |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) |
| Cellular Localization | Secreted , extracellular space |
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000
Application
Antithrombin III antibody detects Antithrombin III protein at cytoplasm and nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. Antithrombin III antibody diluted at 1:250.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Antithrombin III antibody at 1:500 dilution.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
