Application
Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using AP2B1 antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:1000| Product Name | AP2B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AP2B1 (AP-2 complex subunit beta)The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AP2B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, BovineRat (100%), Zebrafish (99%), Xenopus laevis (98%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADTB2 antibody, AP105B antibody, AP2-BETA antibody, CLAPB1 antibody, DKFZp781K0743 antibody, AP2B1 antibody, "clathrin-associated/assembly/adaptor protein, large, beta 1 antibody", adapter-related protein complex 2 beta subunit antibody, AP-2 complex subun |
|---|---|
| Molecular Weight(MW) | 105 kDa (note) (2) |
| Cellular Localization | Cell membrane , Membrane , coated pit |
Application
Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using AP2B1 antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:1000| Positive Control | IMR32 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
