Application
Sample(30 μg of whole cell lysate) A:293T B:HeLa S3 10% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using AP2M1 antibody at 1:100 dilution.| Product Name | AP2M1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AP2M1 (adaptor-related protein complex 2, mu 1 subunit)This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AP2M1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (100%), Rat (100%), Xenopus laevis (99%), Dog (100%), Pig (99%), Chicken (97%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AP50 antibody, CLAPM1 antibody, mu2 antibody, AP2M1 antibody, "clathrin-associated/assembly/adaptor protein, medium 1 antibody", plasma membrane adaptor AP-2 50kDA protein antibody, AP-2 mu chain antibody, adaptor protein complex AP-2 subunit mu antibody, |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (3) |
| Cellular Localization | Cell membrane , Membrane , coated pit |
Application
Sample(30 μg of whole cell lysate) A:293T B:HeLa S3 10% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using AP2M1 antibody at 1:100 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
