Application
Immunofluorescence analysis of methanol-fixed A431, using APC antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using APC antibody at 1:500 dilution.Application
APC antibody [C3], C-term detects APC protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APC protein stained by APC antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Product Name | APC antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APC (adenomatous polyposis coli)This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human APC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (94%), Rat (94%), Pig (88%), Bovine (88%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BTPS2 antibody, DP2 antibody, DP2.5 antibody, DP3 antibody, GS antibody, APC antibody, deleted in polyposis 2.5 antibody, "protein phosphatase 1, regulatory subunit 46 antibody", adenomatous polyposis coli protein antibody, adenomatosis polyposis coli tumo |
|---|---|
| Molecular Weight(MW) | 312 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed A431, using APC antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using APC antibody at 1:500 dilution.
Application
APC antibody [C3], C-term detects APC protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APC protein stained by APC antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
