Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:1000| Product Name | APPL1 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APPL1 (adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1)The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human APPL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Pig, BovineXenopus laevis (89%), Pig (97%), Bovine (98%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APPL antibody, DIP13alpha antibody, APPL1 antibody, DCC-interacting protein 13-alpha antibody, "adaptor protein containing pH domain, PTB domain and leucine zipper motif 1 antibody", "adapter protein containing PH domain, PTB domain and leucine zipper moti |
|---|---|
| Molecular Weight(MW) | 80 kDa (note) (2) |
| Cellular Localization | Early endosome membrane , Nucleus |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
