Application
Immunohistochemical analysis of paraffin-embedded AZ521 xenograft, using APPL1 antibody at 1:100 dilution.Application
APPL1 antibody [N1N2], N-term detects APPL1 protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. APPL1 antibody [N1N2], N-term dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000| Product Name | APPL1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APPL1 (adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1)The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human APPL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, BovineZebrafish (86%), Xenopus laevis (89%), Pig (98%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APPL antibody, DIP13alpha antibody, APPL1 antibody, DCC-interacting protein 13-alpha antibody, "adaptor protein containing pH domain, PTB domain and leucine zipper motif 1 antibody", "adapter protein containing PH domain, PTB domain and leucine zipper moti |
|---|---|
| Molecular Weight(MW) | 80 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded AZ521 xenograft, using APPL1 antibody at 1:100 dilution.
Application
APPL1 antibody [N1N2], N-term detects APPL1 protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. APPL1 antibody [N1N2], N-term dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000| Positive Control | U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
