Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using ARD1A antibody at 1:500 dilution.Application
ARD1A antibody detects ARD1A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and blotted with ARD1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of ARD1A protein from HeLa whole cell extracts using 5 μg of ARD1A antibody . Western blot analysis was performed using ARD1A antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
ARD1A antibody detects NAA10 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: NAA10 protein stained by ARD1A antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample (30 µg of whole cell lysate) A: H1299 transfected scramble siRNA B: H1299 transfected ARDIA siRNA 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ARD1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARD1A (N(alpha)-acetyltransferase 10, NatA catalytic subunit)N-alpha-acetylation is one of the most common protein modifications that occurs during protein synthesis and involves the transfer of an acetyl group from acetyl-coenzyme A to the protein alpha-amino group. ARD1A, together with NATH (NARG1; MIM 608000), is part of a major N-alpha-acetyltransferase complex responsible for alpha-acetylation of proteins and peptides (Sanchez-Puig and Fersht, 2006 [PubMed 16823041]).[supplied by OMIM] |
| Immunogen | Full length human ARD1A Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Rabbit, BovineRat (91%), Xenopus laevis (82%), Rabbit (92%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARD1 antibody, ARD1A antibody, DXS707 antibody, MGC71248 antibody, TE2 antibody, NAA10 antibody, N-alpha-acetyltransferase 10 antibody, N-terminal acetyltransferase complex ARD1 subunit homolog A antibody, "N-alpha-acetyltransferase 10, NatA catalytic subu |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using ARD1A antibody at 1:500 dilution.
Application
ARD1A antibody detects ARD1A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and blotted with ARD1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of ARD1A protein from HeLa whole cell extracts using 5 μg of ARD1A antibody . Western blot analysis was performed using ARD1A antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
ARD1A antibody detects NAA10 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: NAA10 protein stained by ARD1A antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample (30 µg of whole cell lysate) A: H1299 transfected scramble siRNA B: H1299 transfected ARDIA siRNA 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | H1299 transfected scramble siRNA , H1299 transfected ARDIA siRNA , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
