Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ARHGEF3 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ARHGEF3 antibody [N1C1] detects ARHGEF3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ARHGEF3 protein stained by ARHGEF3 antibody [N1C1] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Product Name | ARHGEF3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARHGEF3 (Rho guanine nucleotide exchange factor (GEF) 3)Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARHGEF3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, ChickenMouse (100%), Rat (99%), Zebrafish (87%), Chicken (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434F2429 antibody, FLJ98126 antibody, GEF3 antibody, MGC118905 antibody, STA3 antibody, XPLN antibody, ARHGEF3 antibody, "exchange factor found in platelets and leukemic and neuronal tissues, XPLN antibody", rho guanine nucleotide exchange factor 3 an |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (5) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using ARHGEF3 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ARHGEF3 antibody [N1C1] detects ARHGEF3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ARHGEF3 protein stained by ARHGEF3 antibody [N1C1] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
