Application
Western blot analysis of AS160 (pT642) expression in LO2 insulin-treated (A); Raw264.7 insulin-treated (B); PC12 insulin-treated (C); NIH3T3 UV-treated (D) whole cell lysates.Application
Immunofluorescent analysis of AS160 (pT642) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).Application
Immunohistochemical analysis of AS160 (pT642) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.IF
(d) The relative phosphorylation of AS160 was quantified by Immunofluorescence staining.| Product Name | AS160 / TBC1D4 (phospho Thr642) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AS160 / TBC1D4 (phospho Thr642) |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AS160 / TBC1D4. The exact sequence is proprietary. |
| Modification | p-Thr642 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TBC1D4 TBC1 domain family member 4 AS160 |
|---|---|
| Target | AS160 / TBC1D4 |
| Tissue Specificity | Recognizes endogenous levels of AS160 (pT642) protein. |
Application
Western blot analysis of AS160 (pT642) expression in LO2 insulin-treated (A); Raw264.7 insulin-treated (B); PC12 insulin-treated (C); NIH3T3 UV-treated (D) whole cell lysates.
Application
Immunofluorescent analysis of AS160 (pT642) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Application
Immunohistochemical analysis of AS160 (pT642) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF
(d) The relative phosphorylation of AS160 was quantified by Immunofluorescence staining.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
