Application
Western blot analysis of TBC1D4 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). TBC1D4 (arrow) was detected using the purified Pab.Application
TBC1D4 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TBC1D4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | AS160 / TBC1D4 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AS160 / TBC1D4 (N-term)May act as a GTPase-activating protein for RAB2A, RAB8A, RAB1 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. |
| Immunogen | KLH conjugated synthetic peptide between 179~209 amino acids from the N-terminal region of human AS160 / TBC1D4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIAA0603 Akt substrate of 160 kDa TBC1 domain family member 4 TBC1D4 TBC1 domain family member4 Akt substrate of160 kDa AS160 |
|---|---|
| Target | AS160 / TBC1D4 |
| Molecular Weight(MW) | 146.563 kDa (note) |
| Cellular Localization | Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells. |
Application
Western blot analysis of TBC1D4 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). TBC1D4 (arrow) was detected using the purified Pab.
Application
TBC1D4 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TBC1D4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
