Application
Immunofluorescence staining of methanol-fixed Hela cells using ASK1 Antibody .Application
Western blot analysis of extracts from MDA-MB- 435 cells using ASK1 (Ab-83) Antibody and the same antibody preincubated with blocking peptide.Application
Western blot analysis of extracts from MDA-MB- 435 cells using ASK1 Antibody and the same antibody preincubated with blocking peptide.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | ASK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ASK1Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. |
| Immunogen | Peptide sequence around aa.81~85 (G-S-S-V-G) derived from Human ASK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ASK1 MAPKKK5 MEKK5 MAP3K5 mitogen-activated protein kinase kinase kinase 5 apoptosis signal regulating kinase 1 MAP/ERK kinase kinase 5 MEK kinase 5 MAPK/ERK kinase kinase 5 ASK-1 MEKK 5 apoptosis signal-regulating kinase 1 |
|---|---|
| Target | ASK1 |
| Molecular Weight(MW) | 155 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using ASK1 Antibody .
Application
Western blot analysis of extracts from MDA-MB- 435 cells using ASK1 (Ab-83) Antibody and the same antibody preincubated with blocking peptide.
Application
Western blot analysis of extracts from MDA-MB- 435 cells using ASK1 Antibody and the same antibody preincubated with blocking peptide.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
