Application
Western blot analysis of anti-ATXN1 Antibody (S776) in CEM cell line lysates (35ug/lane). ATXN1 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of ATXN1 Antibody (S776) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | Ataxin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Ataxin 1The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 41-81 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 761~791 amino acids surrounding S776 of human ATXN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Ataxin1 SCA1 Spinocerebellar ataxia type 1 protein ATX1 ATXN1 Spinocerebellar ataxia type1 protein Ataxin-1 Ataxin 1 |
|---|---|
| Target | ATXN1 |
| Molecular Weight(MW) | 86.923 kDa (note) |
| Cellular Localization | Colocalizes with USP7 in the nucleus. |
Application
Western blot analysis of anti-ATXN1 Antibody (S776) in CEM cell line lysates (35ug/lane). ATXN1 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of ATXN1 Antibody (S776) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
