Application
Western blot of human melanoma cells incubated with varying doses of the radiometric drug NCS showing phospho-specific immunolabeling of the ATF2 protein phosphorylated at Ser 490, 498 using ATF2 (phospho Ser490/498) antibody .| Product Name | ATF2 (phospho Ser490/498) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATF2 (phospho Ser490/498)Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498 conjugated to KLH |
| Modification | p-Ser490/498 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC111558 CREB2 TREB7 CRE-BP1 CREBP1 ATF2 HB16 CRE BP1 ATF2 (phospho S490/S498) ATF2 (phospho Ser490/Ser498) ATF2 (pS490/pS498) ATF2 (pSer490/pSer498) ATF2 phospho S490/S498 ATF2 phospho Ser490/Ser498 Phospho ATF2 (S490/S498) Phospho ATF2 (S |
|---|---|
| Target | ATF2 (phospho Ser490/498) |
| Molecular Weight(MW) | 74 (note) |
Application
Western blot of human melanoma cells incubated with varying doses of the radiometric drug NCS showing phospho-specific immunolabeling of the ATF2 protein phosphorylated at Ser 490, 498 using ATF2 (phospho Ser490/498) antibody .| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
