Application
Western blot analysis of extract from HeLa cells, using ATF-2 (phospho-Thr71/53) antibody.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF-2 (phospho-Thr71/53) antibody.| Product Name | ATF2 (phospho Thr71/Thr53) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATF2 (phospho T71/T53)This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq] |
| Modification | p-Thr71/Thr53 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | activating transcription factor 2 CRE-BP1 MGC111558 CREBP1 CREB2 TREB7 ATF2 HB16 activating transcription factor2 |
|---|---|
| Target | ATF2 (phospho T71 or T53) |
Application
Western blot analysis of extract from HeLa cells, using ATF-2 (phospho-Thr71/53) antibody.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF-2 (phospho-Thr71/53) antibody.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
