Application
Immunofluorescence staining of methanol-fixed Hela cells using ATF2 (Phospho-Thr73 or 55) Antibody .Application
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using ATF2 (Phospho-Thr71 or 53) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Thr73 or 55) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | ATF2 (phospho Thr73 or 55) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATF2 (Phospho Thr73 or 55)Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 73 or 55 (T-P-T(p)-R-F) derived from Human ATF2. |
| Modification | p-Thr73 or 55 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CRE-BP1 CREB2 HB16 MGC111558 TREB7 ATF2 CREB-2 cAMP-dependent transcription factor ATF-2 cyclic AMP-responsive element-binding protein 2 cAMP response element-binding protein CRE-BP1 cAMP-responsive element-binding protein 2 cyclic AMP-dependent |
|---|---|
| Target | ATF2 (Phospho Thr73 or 55) |
| Molecular Weight(MW) | 65-75 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using ATF2 (Phospho-Thr73 or 55) Antibody .
Application
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using ATF2 (Phospho-Thr71 or 53) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Thr73 or 55) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
