Application
Immunofluorescence staining of methanol-fixed Hela cells using ATF2 Antibody .Application
Western blot analysis of extracts from HT29 and K562 cells using ATF2 Antibody .Application
Western blot analysis of extracts from HT29 and K562 cells using ATF2(Ab-71 or 53) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | ATF2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATF2Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex. |
| Immunogen | Peptide sequence around aa.69~73 or 51~55 (T-P-T-P-T) derived from Human ATF2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CRE-BP1 CREB2 HB16 MGC111558 TREB7 ATF2 CREB-2 cAMP-dependent transcription factor ATF-2 cyclic AMP-responsive element-binding protein 2 cAMP response element-binding protein CRE-BP1 cAMP-responsive element-binding protein 2 cyclic AMP-dependen |
|---|---|
| Target | ATF2 |
| Molecular Weight(MW) | 65-75 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using ATF2 Antibody .
Application
Western blot analysis of extracts from HT29 and K562 cells using ATF2 Antibody .
Application
Western blot analysis of extracts from HT29 and K562 cells using ATF2(Ab-71 or 53) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
