Application
ATF3 antibody detects ATF3 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells, irradiated (right) or non-irradiated (left) with 100 J/m2 UVC with 8hr recovery, were fixed in 4% paraformaldehyde at RT for 5 min. Green: ATF3 protein stained by ATF3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Wild-type (WT) and ATF3 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATF3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ATF3 antibody detects ATF3 protein by western blot analysis. A HCT116 (untreated) B HCT116 (UVC-treated 10 J/m2 and recover for 8 hr) 12 % SDS-PAGE ATF3 antibody dilution: 1:1000| Product Name | ATF3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATF3 (activating transcription factor 3)Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. [provided by RefSeq] |
| Immunogen | Full length human ATF3 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rhesus Monkey, BovineMouse (95%), Rat (95%), Xenopus laevis (80%), Rhesus Monkey (99%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | activating transcription factor 3 |
|---|---|
| Target | ATF3 |
| Cellular Localization | Nucleus |
Application
ATF3 antibody detects ATF3 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells, irradiated (right) or non-irradiated (left) with 100 J/m2 UVC with 8hr recovery, were fixed in 4% paraformaldehyde at RT for 5 min. Green: ATF3 protein stained by ATF3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Wild-type (WT) and ATF3 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATF3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ATF3 antibody detects ATF3 protein by western blot analysis. A HCT116 (untreated) B HCT116 (UVC-treated 10 J/m2 and recover for 8 hr) 12 % SDS-PAGE ATF3 antibody dilution: 1:1000| Positive Control | HCT116(UVC-treated 10 J/m2 and recover for 8hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
