Application
Western blot analysis of ATF6 Antibody (Center) in A2058 cell line lysates (35ug/lane). ATF6 (arrow) was detected using the purified Pab.Application
ATF6 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ATF6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ATF6 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ATF6 (Internal)ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.[supplied by OMIM] |
| Immunogen | KLH conjugated synthetic peptide between 356~386 amino acids from the Center region of human ATF6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000. For IHC-P: Use at a dilution of 1:10-50. For FACS: Use at a dilution of 1:10-50. Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATF6alpha ATF6-alpha ATF6 Processed cyclic AMPdependent transcription factor ATF6 alpha Activating transcription factor6 alpha Processed cyclic AMP-dependent transcription factor ATF-6 alpha cAMP-dependent transcription factor ATF-6 alpha C Activat |
|---|---|
| Target | ATF6 |
| Molecular Weight(MW) | 74.585 kDa (note) |
| Cellular Localization | Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. |
Application
Western blot analysis of ATF6 Antibody (Center) in A2058 cell line lysates (35ug/lane). ATF6 (arrow) was detected using the purified Pab.
Application
ATF6 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ATF6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000. For IHC-P: Use at a dilution of 1:10-50. For FACS: Use at a dilution of 1:10-50. Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
