Application
Immunoprecipitation of ATG10 protein from SKNSH whole cell extracts using 5 μg of ATG10 antibody . Western blot analysis was performed using ATG10 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
ATG10 antibody detects ATG10 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 whole cell lysate/extract of human ATG10-transfected 293T cells 12 % SDS-PAGE ATG10 antibody dilution: 1:5000| Product Name | ATG10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Atg10 (autophagy related 10)Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM] |
| Immunogen | Full length human ATG10 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | pp12616 ATG10 APG10 autophagy related 10 APG10L |
|---|---|
| Target | Atg10 |
| Molecular Weight(MW) | 25 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunoprecipitation of ATG10 protein from SKNSH whole cell extracts using 5 μg of ATG10 antibody . Western blot analysis was performed using ATG10 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
ATG10 antibody detects ATG10 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 whole cell lysate/extract of human ATG10-transfected 293T cells 12 % SDS-PAGE ATG10 antibody dilution: 1:5000| Positive Control | human Atg10-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
