Application
Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 ug/mL.Application
Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 ug/mL.| Product Name | ATG12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG12Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008] |
| Immunogen | ATG12 antibody was raised against a 15 amino acid synthetic peptide from near the center of human ATG12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 0.5 - 1 ug/ml. IHC-P: 2.5 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APG12 APG12L autophagy related 12 FBR93 HAPG12 |
|---|---|
| Target | ATG12 |
Application
Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 ug/mL.
Application
Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 ug/mL.| Application Notes | WB: 0.5 - 1 ug/ml. IHC-P: 2.5 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
