Application
IHC-P analysis of human colon tissue using Apg12 antibody [EPR4799] at a dilution of 1:50.Application
WB analysis of lysates from (1) Apg12-transfected and (2) non-transfected 293T cells (10 μg per lane) using Apg12 antibody [EPR4799] at a dilution of 1:1,000.| Product Name | ATG12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR4799] to ATG12Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008] |
| Immunogen | A synthetic peptide corresponding to residues in Human Apg12. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR4799 |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FBR93 ATG12 autophagy related 12 HAPG12 APG12L APG12 |
|---|---|
| Target | ATG12 |
Application
IHC-P analysis of human colon tissue using Apg12 antibody [EPR4799] at a dilution of 1:50.
Application
WB analysis of lysates from (1) Apg12-transfected and (2) non-transfected 293T cells (10 μg per lane) using Apg12 antibody [EPR4799] at a dilution of 1:1,000.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for 12 months at -20°C |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
