Application
Immunohistochemistry of ATG13 in mouse heart with ATG13 antibody at 5 ug/ml.Application
Immunofluorescence of ATG13 in Mouse Heart cells with ATG13 antibody at 20 ug/mL.Application
Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | ATG13 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATG13Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Autophagyrelated protein 13 Autophagyrelated protein13 Autophagy-related protein 13 ATG13 Autophagy related protein 13 |
|---|---|
| Target | ATG13 |
| Tissue Specificity | This antibody is specific for ATG13 |
Application
Immunohistochemistry of ATG13 in mouse heart with ATG13 antibody at 5 ug/ml.
Application
Immunofluorescence of ATG13 in Mouse Heart cells with ATG13 antibody at 20 ug/mL.
Application
Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
