Application
Western blot of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using antibody at 2 ug/mlApplication
Human Heart (formalin-fixed, paraffin-embedded) stained with ATG4B antibody at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Product Name | ATG4B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATG4BAutophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Synthetic peptide - KLH conjugated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB (1 - 3 µg/ml), IHC-P (10 µg/ml): |
|
| Species Reactivity | HumanMouseChickenRatZebra FishPuffer Fish(Fugu)Xenopus |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | APG4B AUTL1 MGC1353 |
|---|---|
| Target | ATG4B |
| Tissue Specificity | A portion of amino acid 350-400 of human APG4B |
Application
Western blot of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using antibody at 2 ug/ml
Application
Human Heart (formalin-fixed, paraffin-embedded) stained with ATG4B antibody at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Application Notes | WB (1 - 3 µg/ml), IHC-P (10 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Storage Buffer | PBS, 0.2% gelatin, 0.05% sodium azide. |
