Application
Immunofluorescence of APG7 in MCF7 cells with APG7 antibody at 20 μg/mL.Application
Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunocytochemistry of APG7 in MCF7 cells with APG7 antibody at 10 μg/mL.| Product Name | ATG7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG7This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009] |
| Immunogen | Predicted: 69, 74, 77 kDa Observed: 77 kDa |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB: 0.5 - 2 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APG7-LIKE autophagy related 7 GSA7 APG7L |
|---|---|
| Target | ATG7 |
Application
Immunofluorescence of APG7 in MCF7 cells with APG7 antibody at 20 μg/mL.
Application
Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunocytochemistry of APG7 in MCF7 cells with APG7 antibody at 10 μg/mL.| Application Notes | WB: 0.5 - 2 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stored At 4ºc For Three Months And -20ºc, Stable For Up To One Year. As With All Antibodies Care Should Be Taken To Avoid Repeated Freeze Thaw Cycles. Antibodies Should Not Be Exposed To Prolonged High Temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
