Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody diluted at 1:1000.Application
Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody diluted at 1:1000.| Product Name | ATM (phospho Ser1981) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATM (phospho Ser1981) (ataxia telangiectasia mutated)The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ATM (phospho Ser1981). The exact sequence is proprietary. |
| Modification | p-Ser1981 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | phospho S1981 ATM phospho ATM ATM phospho S1981 ATM serine/threonine kinase phospho ATM (S1981) ATM phospho Ser1981 ATE ATD ATC ATM phospho ATM (pSer1981) TELO1 phospho ATM (pS1981) ATM (pS1981) ATA AT1 TEL1 |
|---|---|
| Target | ATM (phospho Ser1981) |
| Molecular Weight(MW) | 351 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasmic vesicle |
Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody diluted at 1:1000.
Application
Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody diluted at 1:1000.| Positive Control | HCT116 (UVC 100J/m2, recover 6hr) , 293T (100J/m2 UVC and recover for 4hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
