Application
Immunohistochemical staining usingApplication
Immunohistochemistry: ATM Antibody - Staining of Testis, Seminiferous Tubule.Application
Western Blot: ATM Antibody - Detection of ATM in HeLa nuclear extract using . (30 sec. exposure)Application
Western Blot: ATM Antibody - Detection of ATM in Raji whole cell lysate.| Product Name | ATM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ATMAtaxia-telangiectasia (A-T) is a recessive childhood disease caused by mutations in the ATM (AT-mutated) gene. Symptoms include neurological abnormalities that cause unsteady posture, dilated blood vessels, infertility, radiation sensitivity, immune deficiencies and lymphoid malignancies. It appears that the diverse defects seen in ATM null mammals are manifestations of disparate signal transduction defects. The ATM protein is related to a family of proteins through a c-terminal phoshatidylinositol 3-kinase (PI3-kinase) domain. Members of the PI3-kinase family of proteins are involved in cell cycle control, DNA replication, recombination and repair. ATM also shares sequence homology with portions of the yeast RAD3 gene. The main role of ATM appears to be induction of a DNA-damage control pathway in response to genotoxic insults, such as ionizing radiation or anti-tumor medications and the programmed DNA breaks of meiosis. |
| Immunogen | A fragment of human ATM protein expressed in E.coli. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| For IHC: Use at a dilution of 1:750. For WB: Use at a dilution of 1:2,500. For IP: Use at a dilution of 1:333. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATD 114480 ATA AT1 ATC ATE DKFZp781A0353 472 ATM TEL1 ATDC Q13315 MGC74674 |
|---|---|
| Target | ATM |
| Tissue Specificity | This antibody is specific for ATM. |
| Cellular Localization | Nuclear staining is seen. |
Application
Immunohistochemical staining using
Application
Immunohistochemistry: ATM Antibody - Staining of Testis, Seminiferous Tubule.
Application
Western Blot: ATM Antibody - Detection of ATM in HeLa nuclear extract using . (30 sec. exposure)
Application
Western Blot: ATM Antibody - Detection of ATM in Raji whole cell lysate.| Application Notes | For IHC: Use at a dilution of 1:750. For WB: Use at a dilution of 1:2,500. For IP: Use at a dilution of 1:333. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline with 50% glycerol containing 0.05% sodium azide. |
