Application
Immunofluorescence staining of methanol-fixed Hela cells using ATM Antibody .Application
Western blot analysis of extracts from 293T cells using ATM Antibody and the same antibody preincubated with blocking peptide.| Product Name | ATM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATMATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Peptide sequence around aa.1979~1983 (E-G-S-Q-S) derived from Human ATM. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AT1 ATA ATC ATD ATDC ATE DKFZp781A0353 MGC74674 TEL1 TELO1 ATM AT mutated TEL1 telomere maintenance 1 homolog A-T mutated serine-protein kinase ATM ataxia telangiectasia mutated |
|---|---|
| Target | ATM |
| Molecular Weight(MW) | 350 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using ATM Antibody .
Application
Western blot analysis of extracts from 293T cells using ATM Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
